primary antibodies against sh2d4a  (Proteintech)

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 1. SH2D4A was downregulated in ESCC and lower expression of SH2D4A resulted in an adverse clinical outcome. (A) The mRNA expression of SH2D4A was reduced in GSE53625. (B) The mRNA expression of SH2D4A was reduced in 16 pairs of ESCC tissues detected by RT-qPCR. (C) The expression of SH2D4A were downregulated in ESCC tissues. (D) The relative mRNA expression of SH2D4A was reduced in esophageal carcinoma cell lines detected by RT-qPCR (n = 3 per group). (E) The expression of SH2D4A were downregulated in esophageal carcinoma cell lines. (F, G) Kaplan-Meier analysis of DFS and OS in patients diagnosed with ESCC. All data were provided as mean ± SD. * P < 0.05, and *** P < 0.001.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: Expressing, Quantitative RT-PCR

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 2. SH2D4A overexpression was correlated with a reduction of the proliferation and migration in esophageal carcinoma cells. (A, B) The efficiency of SH2D4A overexpression conducted by lentivirus infection in KYSE-150 and TE-10 (n = 3 per group). (C, D, E) CCK-8 assay, colony formation assay and EDU revealed that SH2D4A overexpression reduce the ability of cells to proliferate (n = 3 per group). (F, G) Transwell migration assay and wound healing assay revealed that SH2D4A overexpression reduce the ability of cells to migrate (n = 3 per group). Scale bar = 100 μm. All data were provided as mean ± SD. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: Over Expression, Migration, Infection, CCK-8 Assay, Colony Assay, Transwell Migration Assay, Wound Healing Assay

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 3. SH2D4 A knockdown promoted the proliferation and migration of esophageal carcinoma cells. (A, B) The efficiency of SH2D4 A knockdown conducted by lentivirus infection in Eca109 and TE-1 (n = 3 per group). (C, D, E) CCK-8 assay, colony formation assay and EDU revealed that SH2D4A knockdown enhance the ability of cells to proliferate (n = 3 per group). (F, G) Transwell migration assay and wound healing assay revealed that SH2D4A knockdown enhance the ability of cells to migrate (n = 3, per group). Scale bar = 100 μm. All data were provided as mean ± SD. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: Knockdown, Migration, Infection, CCK-8 Assay, Colony Assay, Transwell Migration Assay, Wound Healing Assay

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 4. SH2D4A regulated FAK/PI3K/AKT signaling pathway in esophageal cancer cells. (A) SH2D4A was enriched in focal adhesion assemblies through KEGG pathway analysis. (B) Correlation analysis between SH2D4A and related molecules in FAK/PI3K/AKT signaling pathway signaling pathway. (C, D) Expression of FAK/PI3K/AKT signaling pathway-related proteins were determined in different cell lines by western blot.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: Expressing, Western Blot

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 5. SH2D4A inhibited esophageal carcinoma cells from proliferating and migrating through the FAK/PI3K/AKT signaling pathway. (A, B, C) CCK-8 assay, colony formation assay and EDU indicated that PF562271 reduced cell proliferation caused by SH2D4A knockdown (n = 3 per group). (D, E) Transwell migration assay and wound healing assay indicated that PF562271 reduced cell migration caused by SH2D4A knockdown (n = 3 per group). (F) PF562271 reduced the facilitation of FAK/PI3K/AKT signaling pathway caused by SH2D4A knockdown. Scale bar = 100 μm. All data were provided as mean ± SD. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: CCK-8 Assay, Colony Assay, Knockdown, Transwell Migration Assay, Wound Healing Assay, Migration

Journal: Cellular signalling

Article Title: SH2D4A inhibits esophageal squamous cell carcinoma progression through FAK/PI3K/AKT signaling pathway.

doi: 10.1016/j.cellsig.2023.110997

Figure Lengend Snippet: Fig. 6. SH2D4A knockdown promoted ESCC tumorigenesis in vivo. (A) Tumor sizes were bigger in the si-SH2D4A group than in the si-NC group (n = 5 mice). (B) Growth rate was more rapid in the si-SH2D4A group than in the si-NC group (n = 5 mice). (C) Tumors are heavier in the si-SH2D4A group than in the si-NC group (n = 5 mice). (D) Expression of Ki67 and SH2D4A in tumor tissues were measured by immunohistochemistry. All data were provided as mean ± SD. ** P < 0.01.

Article Snippet: Primary antibodies against SH2D4A (15957–1-AP, 1:1000) were purchased from Proteintech.

Techniques: Knockdown, In Vivo, Expressing, Immunohistochemistry